The laboratory uses state of the art techniques in physiology, histopathology, biochemistry, Immunology and molecular biology to investigate the end points of experiments.
The laboratory uses histopathological stains such as Hematoxylin-Eosin, Oil-Red-O, Picrosirius red and Trichrome Masson for its studies. The University core faciliries are utilized for tissue processing and slide preparations.
Enzyme assays, kinetics, free radical adduct formation assays and serum biochemical assays for disease markers are carried out in the laboratory.
The laboratory specializes on immunological techniques such as immunohistochemistry, immunoprecipitation, Enzyme-linked immunosorbent assays (ELISAs), immunoblots and immune cell isolations (using core facilities).
Currently the laboratory uses state of the art techniques like quantitative real time PCR for estimation of mrna expression, PCR amplifications of methylated DNA, micro RNA analysis and chromatin immunoprecipitation.
Human and animal tissue culture
The laboratory uses human and mouse cell lines and primary cells for studying the molecular mechanisms of the disease process. Prominent cell lines used are HepG2, Raw 264.7, LX-2, 8B and primary cells of human and mouse origin (hepatocytes, Kupffer cells and stellate cells).
Our Laboratory in conjunction with the School of Medicine, analyses the changes in the gut micro-biome due to metabolic syndrome caused by Non Alcoholic Fatty liver diseases.